【 Testing purpose 】
Feline leukemia virus (FeLV) is a retrovirus that is widespread in the world. Cats infected with the virus have a greatly increased risk of lymphoma and other tumours; The virus can cause coagulation abnormalities or other blood disorders such as regenerative/non-regenerative anemia; It can also lead to the collapse of the immune system, leading to hemolytic anemia, glomerulonephritis, and other diseases.
【 Detection principle 】
Products were quantified for FeLV in cat serum/plasma using fluorescence immunochromatography. Basic principle: The nitrocellulose membrane is marked with T and C lines, respectively, and the T line is marked with antibody A, which specifically recognizes FeLV antigen. The binding pad was sprayed with anti-B labeled with another fluorescent nanomaterial capable of specifically recognizing FeLV. The FeLV in the sample was first bound to the antibody B labeled with the nano-material to form a complex, and then it was precipitated to the upper layer. The complex and T-line antibody A were combined to form a sandwich structure. When the excitation light was irradiated, the nano-material emitted a fluorescence signal, and the signal intensity was positively correlated with the FeLV concentration in the sample. Therefore, reliable and effective detection plays a positive guiding role in prevention, diagnosis and treatment.
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