Feline Respiratory Pathogen Pentaplex Nucleic Acid Detection Kit

【Background】
Feline upper respiratory tract disease (FURD) is one of the most important causes of morbidity and mortality in young cats. The typical clinical symptoms of FURD are fever, decreased appetite, depression, serous, mucous or purulent secretions in the eyes and nasal cavity, edema or ulceration in the oropharynx, salivation, and occasional cough and sneezing. The common pathogens were feline calicivirus (FCV), feline herpesvirus type 1 (FHV-I), Mycoplasma (M. felis), Chlamydia felis (C. felis) and Bordetella bronchiseptica (Bb).

【Principle of test procedure】
Feline Respiratory Pathogen Pentaplex Nucleic Acid Detection Kit is an in vitro nucleic acid amplification test for the nucleic acid of FHV-1, M. felis, FCV, Bordetella bronchiseptica (Bb) and C. felis.
The lyophilized reagent contains specific primer pairs, probes, reverse transcriptase, DNA polymerase, dNTPs, surfactant, buffer and lyoprotectant.
This test is based on three major processes: (1) automated sample preparation to extract total nucleic acid of specimen by AIMDX 1800VET; (2) reverse transcription of the target RNA to generate complementary DNA (cDNA); (3) PCR amplification of target cDNA using specific complementary primers, and simultaneous detection of cleaved TaqMan probes that permit detection of amplified product of targets.