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Canine Cortisol Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)（cCor）
Test Purpose
Canine Cortisol (cCortisol) is a steroid hormone produced by the canine adrenal cortex. Cortisol helps maintain a constant blood sugar level, reduces inflammation and helps the body manage stress. While various conditions caused by abnormally high levels of the alcohol hormone are called Cushing syndrome (CS), both dogs and cats can suffer from CS, which is more common in dogs than in cats. Dogs of middle and old age (about 7 to 12 years old)
are most likely to develop the disease. The disease develops slowly and early symptoms are not easy to detect. CS can be clinically diagnosed by adrenocorticotropic hormone (ACTH) stimulation test and dexamethasone suppression test, and its different types: adrenal-dependent (ATH) and pituitary-dependent (PDH).
Detection Principle
This product uses fluorescence immunochromatography to quantitatively detect the content of cCortisol in dog serum/plasma. Basic principle: T and C lines are marked on the nitrocellulose membrane, the T line is coated with cCortisol antigen a, and the binding pad is sprayed with a fluorescent nanomaterial labeled antibody b that can specifically recognize cCortisol.
he cCortisol in the sample is first labeled with the nanomaterial. The antibody b binds to form a complex, and then chromatographs upward. The complex competes with T-line antigen a and cannot be captured; On the contrary, when there is no cCortisol in the sample, antibody b binds to antigen a. When the excitation light is irradiated, the nano material emits a fluorescent signal, and the strength of the signal is inversely proportional to the concentration of cCortisol in the sample.
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