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Feline Total lgE Quantitative Kit（Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)（fTIgE）
Purpose of Detection
IgE is a class of immunoglobulin (Ig) with a molecular weight of 188kD and very low content in serum. It is commonly used In the diagnosis of allergic reaction, in addition, can also assist in the diagnosis of parasitic infection, multiple myeloma. 1. Passing Sensitization: When there is an allergic reaction, resulting in the increase of allergen lgE, the higher the allergen lgE, indicating allergic reaction The more serious it should be. 2. Parasite infection: After the pet is infected with the parasite, the allergen lgE may also increase. It is generally associated with mild allergy caused by insect protein. In addition, the reported occurrence of tumors may also lead to a total IgE is elevated.
Detection Principle
cTIgE content in serum/plasma was quantitatively detected by fluorescence immunochromatography. Basic principles:
T and C lines were drawn on the nitrate fiber membrane respectively, and T lines were coated with antibody a that specifically recognized cTIgE antigen. The pad was sprayed with another fluorescent nanomaterial labeled antibody b, which can specifically recognize cTIgE. cTIgE was first bound to the nanomaterial labeled antibody b to form a complex, and then to the upper layer, the complex and T-line antibody a binds to form sandwich structure. When the excited light irradiates, the nanomaterial emits fluorescence signal.
The strength of the signal is positively correlated with the concentration of cTIgE in the sample.
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