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Feline Pancrelipase Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)（fPL）
Purpose of Detection
Feline pancreatitis is an inflammatory invasive disease of the pancreas. It can be divided into acute pancreatitis and chronic pancreatitis Adenitis. In acute pancreatitis, pancreatic neutrophil infiltration, pancreatic necrosis, peripancreatic fat necrosis, Edema and injury. Fibrosis and atrophy of the pancreas are seen in chronic pancreatitis. Chronic as opposed to acute pancreatitis Pancreatitis is less harmful but more common.
When a cat has pancreatitis, the pancreas is damaged and the amount of pancreatic lipase in the blood increases dramatically. Orders, Previously, pancreatic lipase was one of the best indicators of specificity in the diagnosis of feline pancreatitis.
Detection Principle
Fluorescence immunochromatography was used to quantitatively detect the fPL content in whole blood, serum/plasma. basic Principle: There are T and C lines on the nitric acid fiber membrane respectively, and the T lines are coated with antibodies that specifically recognize fPL antigen A. The binding pad was sprayed with another fluorescent nanomaterial labeled antibody b that can specifically recognize fPL in the sample fPL is first bound to the nanomaterial labeled antibody b to form a complex, which is then chromatography to the upper layer It binds with T-line antibody a to form a sandwich structure. When the excited light irradiates, the nanomaterial emits fluorescence signal. The strength of the signal is positively correlated with the concentration of fPL in the sample.
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