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Feline Pancreas lipase Test Kit (fPL）
Detection Purpose
Feline pancrelipase (fPL) is an inflammatory disease of the pancreas. It is generally divides into acute pancreatitis and chronic pancreatitis. Acute pancreatitis is characterized by neutrophil infiltration in the pancreas, pancreatic necrosis, fat necrosis around the pancreas, edema and tissue injury. Chronic pancreatitis is characterized by pancreatic fibrosis and atrophy. Compared with acute pancreatitis, chronic pancreatitis is less harmful but occurs more frequently.
When a cat has pancreatitis, the pancreas is damaged, and the level of pancreatic lipase in the cat’s blood increase rapidly. Currently, pancreatic lipase is one of the best specific indicators for diagnosing feline pancreatitis.
Detection Principle
This product utilizes fluorescence immunochromatography to quantitatively detect the fPL content in whole blood and serum/plasma. The basic principle is as follows: T and C lines are respectively marked on the nitrocellulose membrane. The T line is coated with an antibody a that specifically recognizes the fPL antigen. The binding pad is sprayed with another fluorescent nanomaterial-labeled antibody b that specifically recognizes fPL. The fPL in the sample first combines with the nanomaterial-labeled antibody b to form a complex. Then, through chromatography, the complex moves upward to binds to the T-line antibody a, forming the sandwich structure. When the test strip is irradiated by excitation light, the nanomaterial emits a fluorescent signal, and the intensity of this signal is positively correlated with the concentration of fPL in the sample.
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