Feline Health Marker Combined Detection（5-6 items）

【 Testing purpose 】
Feline pancreatic lipase (fPL) : The pancreas is the second largest digestive gland in the animal body (the first is the liver), located in the front abdomen of the body, divided into left and right lobes. Its main function is to secrete enzymes necessary for the body.Pancreatitis is divided into acute pancreatitis and chronic pancreatitis. The damage caused by the former is mostly temporary, while the latter leaves permanent fibrosis and atrophy in the course of repeated chronic inflammation. Among them, chronic pancreatitis accounts for about 2/3 of cat pancreatitis.
Cholyglycine (CG) is one of the conjugated cholic acids formed by the combination of cholic acid and glycine. Glycocholic acid is the most important bile acid component in serum during late pregnancy. When liver cells were damaged, the uptake of CG by liver cells decreased, resulting in the increase of CG content in blood. In cholestasis, the excretion of cholic acid by the liver is impaired, and the content of CG returned to the blood circulation is increased, which also increases the content of CG in the blood.Bile acids are stored in the gallbladder, which can be eliminated through the hepatic duct after eating. Similarly, liver diseases and bile duct obstruction may cause the abnormal index.
Cystatin C is one of the cystatin proteins. The most important physiological function is to regulate the activity of cysteine protease, which has the strongest inhibitory effect on cathepsin B, papain, figs protease, and cathepsin H and I released by lysosomes. It plays an important role in the metabolism of intracellular peptides and proteins, especially in the metabolism of collagen, which can hydrolyze some prehormones and release them into target tissues to play their respective biological roles. Hereditary cerebral hemorrhage with amyloidosis is a disease directly related to cystatin C gene mutation, which can cause cerebral vascular rupture, cerebral hemorrhage and other serious consequences. The kidney is the only place to clear circulating cystatin C, and the production of cystatin C is constant. The serum cystatin C level mainly depends on GFR, which is an ideal endogenous marker to reflect the changes of GFR. The changes in the content of other body fluids are also associated with a variety of diseases.
NT-proBNP (N-terminal pro-brain natriuretic peptide), also known as B-type diuretic peptide, is a protein hormone secreted by cardiomyocytes in the ventricles of the heart. When ventricular blood pressure increases, ventricular dilation, myocardial hypertrophy, or pressure on the myocardium increases, the precursor of NT-proBNP, proBNP(consisting of 108 amino acids), is secreted into the bloodstream by cardiomyocytes.
Cat allergen total IgE (fTIgE) :IgE is a kind of immunoglobulin (Ig) with a molecular weight of 188kD and a very low content in serum. It is usually used for the diagnosis of allergic reactions. In addition, it can also assist in the diagnosis of parasitic infections and multiple myeloma. 1. Allergic reaction: when allergic reaction occurs, it leads to the increase of allergen lgE. The higher the allergen lgE, the more serious the allergic reaction is. 2. Parasite infection: after the pet is infected by parasites, the allergen lgE may also increase, which is generally related to mild allergy caused by parasite proteins. In addition, the reported presence of cancer may also contribute to the elevation of total IgE.
【 Detection principle 】
This product uses fluorescence immunochromatography to quantitatively detect the content of fPL/CG/fCysC/fNT-proBNP/fTIgE in cat blood. The basic principle is that the nitrocellulose membrane is marked with T and C lines, and the T line is coated with antibody a that specifically recognizes the antigen. The binding pad is sprayed with another fluorescent nanomaterial labeled antibody b that can specifically recognize the antigen. The antibody in the sample binds to the nanomaterial labeled antibody b to form a complex, which then binds to the T-line antibody A to form a sandwich structure. When the excitation light is irradiated, the nanomaterial emits fluorescent signals. The intensity of the signal was positively correlated with the antigen concentration in the sample.