Feline plague is a severe feline infection caused by feline panleukopenia virus Sexual diseases. The general clinical manifestations are high fever, diarrhea and vomiting, with a high mortality rate, High infectivity and short course of disease, especially in young cats with higher rates of infection and death Rate. Therefore, reliable and effective detection plays a positive guiding role in prevention, diagnosis and treatment.
FPV content in cat feces was quantitatively detected by fluorescence immunochromatography. Basic principles:
Lines T and C were drawn on the nitrate fiber membrane respectively, and T-lines were coated with antibody a that specifically recognized FPV antigen. The binding pad was sprayed with another fluorescent nanomaterial labeled antibody b, which can specifically recognize FPV The FPV is first combined with the nanomaterial labeled antibody b to form a complex, and then goes to the upper layer The complex binds to the T-line antibody a to form a sandwich structure that emits the nanomaterial when stimulated by light The intensity of the fluorescence signal is positively correlated with the concentration of FPV in the sample.
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