Canine thyroid stimulating hormone is produced by glands A hormone secreted by the pituitary gland whose main function is to control and regulate the activity of the thyroid gland. Determination of its concentration is diagnostic And treatment of hyperthyroidism, hypothyroidism one of the important indicators, thyroid function Hypoactive dogs may experience one or more symptoms such as obesity/weight gain, hair loss, and behavioral changes And so on. Therefore, detection of cTSH is of great significance for the diagnosis and treatment of thyroid diseases in dogs Dosimetry provides a convenient way to monitor hypothyroidism in dogs.
cTSH content in serum/plasma was quantitatively detected by fluorescence immunochromatography. Basic principle: nitrate The acid fiber membrane was marked with T and C lines respectively, and the T lines were coated with antibody a that specifically recognized cTSH antigen. In combination with The pad is sprayed with another fluorescent nanomaterial labeled antibody b that specifically recognizes cTSH, cTSH in the sample First, it binds to the nanomaterial labeled antibody b to form a complex. Then, the complex is subjected to the T-line resistance Body a is combined to form a sandwich structure. When the excited light irradiates, the nanomaterial emits a fluorescence signal, and the signal Is positively correlated with cTSH concentration in the sample.
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