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Canine Progesterone Test Kit (cProg)
Detection Purpose
Canine progesterone (cProg) levels in the serum are related to the stage of estrus in female dogs. Compared to LH, cProg concentrations steadily rise during estrus and are easier to track, providing real-time information about the female dog's estrous status. The optimal mating time is typically 3-6 days after the LH peak. The progesterone levels corresponding to the optimal mating time can vary significantly between different dogs, usually ranging from 0 to 50 ng/ml, but values outside this range can also occur. Therefore, combining the degree of keratinization of vaginal epithelial cells with dynamic, real-time monitoring of serum progesterone concentrations can greatly improve the chances of successful conception in female dogs.
Detection Principle
This product is a fluorescent immunochromatographic assay for the quantitative analysis of cProg in canine serum/plasma. Basic principle: T and C lines are drawn on the nitrocellulose membrane, the T line is covered with cProg antigen a, and the binding pad is sprayed with fluorescent nanomaterial-labeled antibody b, which can specifically recognize cProg. cProg in the sample firstly combines with nanomaterial-labeled antibody b to form a complex, and then chromatographed upward, and the complex competes with the T line antigen a, and can not be captured; on the other hand, when there is no cProg in the sample, antibody b binds to antigen a, and when excited light irradiates, the nanomaterial emits fluorescence. On the contrary, when there is no cProg in the sample, the antibody b binds to the antigen a, and the nanomaterials emit a fluorescent signal when the excitation light is irradiated, and the strength of the signal is inversely proportional to the concentration of cProg in the sample.
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