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Canine Progesterone Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)（cProg）
Purpose of Detection
The concentration of canine progesterone in serum is related to the stage of dog estrus. Compared with LH, the concentration of cProg is always on the rise during the female dog's estrus, which is easier to track and can be reversed in real time The best time to breed is 3-6 days after the LH peak, depending on the female dog's estrus condition. Between different females, The level of progesterone corresponding to the optimal mating time varied widely, generally ranging from 0-50ng /ml, but there were also more than that In this range, therefore, the degree of keratinization of the vaginal epithelium was combined with dynamic real-time monitoring of serum progesterone concentrations The evaluation method can greatly improve the probability of conception of female dogs.
Detection Principle
cProg content in dog serum/plasma was quantitatively detected by fluorescence immunochromatography. Basic principle: Fiber nitrate There are T and C lines on the dimensional film respectively, and the T line is coated with cProg antigen a, which can specifically recognize cProg by spraying on the pad
cProg in the sample was first bonded with the nanomaterial labeled antibody b to form Complex, and then to the upper phase, the complex competes with T-line antigen a and cannot be captured; Instead, when there is no sample In the presence of cProg, antibody b binds to antigen a. When the excitation light irradiation, the nanomaterial emits fluorescence signal. The strength of the signal is inversely proportional to the concentration of cProg in the sample.
Test Result
Since optimal progesterone levels are related to breed, age and size of dog, there is no absolute fixed value, the following ranges
For reference only, it is recommended that each laboratory or hospital establish its own reference range according to the clinic
Not in heat: < 1ng/ml;
Estrus: the concentration of progesterone gradually increases, the cycle is generally 7-8 days; In order to improve the success rate of pregnancy, the first progesterone test
The concentration should be within the range of 10-50ng/ml, and it is recommended to breed twice.
10-30ng/ml: first mating within 3h, second mating within 48h;
30-60ng/ml: first mating within 2h and second mating within 24h;
60-80ng/ml: 2h for mating.
The detection range of this kit is 1-80ng/ml. If it exceeds the range, prompt < 1ng/ml, or > 80 ng/ml.
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