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Canine Pancrelipase Quantitative Kit (Fluorescent Immunochromatography Assay of Rare Earth Nanocrystals)（cPL）
Brief Introduction
In most cases of pancreatitis, the underlying cause is usually unknown; But here is an important list of the associated risk factors. Obese animals and those fed a diet high in fat are more likely to develop pancreatitis. Although it is not clear whether hyperlipidemia is a result or part of pancreatitis, it is associated with pancreatitis. Certain breeds of dogs are thought to be prone to pancreatitis, such as mini Chenares or bloodhounds. Many drugs and their family of drugs are also thought to cause pancreatitis in humans, but the evidence for a direct correlation has not been established.
Purpose of Detection
Canine pancreatitis is an inflammatory invasive disease of the pancreas. It can be divided into acute pancreatitis and Chronic pancreatitis. Acute pancreatitis shows neutrophil infiltration, pancreatic necrosis, and pancreas Periglandular fat necrosis, edema and injury. Fibrosis and atrophy of the pancreas are seen in chronic pancreatitis. Compared with acute pancreatitis, chronic pancreatitis is less harmful but more frequent. When dogs suffer from pancreatitis, the pancreas is damaged and the level of pancreatic lipase in the blood increases dramatically High. At present, pancreatic lipase is one of the best indicators to diagnose the specificity of canine pancreatitis.
Detection result
Normal range: < 200 ng/mL
Suspected: 200~400 ng/mL
Positive: ＞400 ng/mL
Detection Principle
cPL content in whole blood, serum/plasma was quantitatively detected by fluorescence immunochromatography. Basic principle: There are T and C lines on the nitrate fiber membrane respectively, and the T lines are coated with specific cPL recognition Antibody a to the antigen. The binding pad is sprayed with another fluorescent nanomaterial label that can specifically recognize cPL The cPL in the sample first binds with the nanomaterial labeled antibody b to form a complex. The complex then binds to the T-line antibody a to form a sandwich structure when the light is excited During irradiation, the nanomaterial emits a fluorescence signal, and the strength of the signal is related to the cPL concentration in the sample Is positively correlated.
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