Canine Health Markers Combined Detection(5-6 items)

【 Testing purpose 】
Canine pancreatic lipase (cPL) : Canine pancreatitis is an inflammatory infiltrative disease of the pancreas. Generally, it can be divided into acute pancreatitis and chronic pancreatitis. Pancreatic neutrophil infiltration, pancreatic necrosis, peripancreatic fat necrosis, edema and injury can be seen in acute pancreatitis. Pancreatic fibrosis and atrophy can be seen in chronic pancreatitis. Compared with acute pancreatitis, chronic pancreatitis is less harmful, but more frequent. When dogs suffer from pancreatitis, the pancreas is damaged, and the level of pancreatic lipase in the blood increases sharply. Currently, pancreatic lipase is one of the best indicators of specificity for the diagnosis of pancreatitis in dogs.
Cholyglycine (CG) is one of the conjugated cholic acids formed by the combination of cholic acid and glycine. Glycocholic acid is the most important bile acid component in serum during late pregnancy. When liver cells were damaged, the uptake of CG by liver cells decreased, resulting in the increase of CG content in blood. In cholestasis, the excretion of cholic acid by the liver is impaired, and the content of CG returned to the blood circulation is increased, which also increases the content of CG in the blood.
Cystatin C is one of the cystatin proteins. So far, Cys C is an endogenous substance that basically meets the requirements of an ideal endogenous GFR marker. It is a sensitive and specific index for the assessment of canine renal function.
N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) is a substance secreted by cardiomyocytes in the Canine ventricle and can be used as a detection index for corresponding heart failure. The concentration of cNT-proBNP in the blood correlates with the severity of the disease. Therefore, NT-proBNP can not only evaluate the severity of acute and chronic heart failure, but also be used as an indicator of its prognosis.
Canine allergen total IgE (cTIgE) :IgE is a kind of immunoglobulin (Ig) with a molecular weight of 188kD and a very low content in serum. It is usually used for the diagnosis of allergic reactions. In addition, it can also assist in the diagnosis of parasitic infections and multiple myeloma. 1. Allergic reaction: when allergic reaction occurs, it leads to the increase of allergen lgE. The higher the allergen lgE, the more serious the allergic reaction is. 2. Parasite infection: after the pet is infected by parasites, the allergen lgE may also increase, which is generally related to mild allergy caused by parasite proteins. In addition, the reported presence of cancer may also contribute to the elevation of total IgE.

【 Detection principle 】
This product uses fluorescence immunochromatography to quantitatively detect the cPL/CG/cCysC/cNT-proBNP/cTIgE content in canine blood. The basic principle is that the nitrocellulose membrane is marked with T and C lines, and the T line is coated with antibody a that specifically recognizes the antigen. The binding pad is sprayed with another fluorescent nanomaterial labeled antibody b that can specifically recognize the antigen. The antibody in the sample binds to the nanomaterial labeled antibody b to form a complex, which then binds to the T-line antibody A to form a sandwich structure. When the excitation light is irradiated, the nanomaterial emits fluorescent signals. The intensity of the signal was positively correlated with the antigen concentration in the sample.