Canine coronavirus is a category of coronaviruses Coronavirus, a genus of toxic family, is a very harmful infectious disease of dogs.
General clinical manifestations are: gastroenteritis symptoms, specifically vomiting, diarrhea and anorexia And so on. Reliable and effective detection plays a positive guiding role in prevention, diagnosis and treatment.
Fluorescence immunochromatography was used for the quantitative detection of CCV in dog feces.
Basic principle: There are T and C lines on the nitric acid fiber membrane respectively, and the T-line coating has specific recognition Antibody a to CCV antigen. The binding pad is sprayed with another fluorescence that can specifically recognize CCV Nanomaterial labeled antibodies from b, samples in CCV first and nanomaterial labeled antibodies b binds to form a complex, and then to the upper chromatography, the complex binds to the T-line antibody A, forming
Sandwich structure, when the excitation light irradiation, the nanomaterial emit fluorescence signal, and the signal The strength is positively correlated with the CCV concentration in the sample.
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